20230608160500 20230608160500 Selcuk Medical Journal sinandemircioglumd@gmail.com Prof. Dr. Sinan DEMİRCİOĞLU Selcuk Medical Journal Selcuk Med J 2149-8059 06 08 2023 39 2 Levent Tümkaya Filiz Mercantepe Aim: The present study aimed to investigate the effects of topiramate on pancreatic islets of Langerhans cells in Methotrexate-related pancreatic injury. Materials and Methods: The study was conducted between 04 July - 24 July 2022 in the Animal Research Unit of Recep Tayyip Erdogan University. Male Spraque-Dawley rats were divided into 3 groups with 8 animals in each group. Control group (C) (Only 0.9% saline was administered). A single dose of 20mg/kg Methotrexate was administered to the MTX group (MTX). The TPM group (TPM) was administered 100 mg/kg/day Topiramate by oral gavage for a total of 14 days, 7 days before and after Methotrexate administration. All subjects were euthanized by anesthesia 16 hours after the l ast Topiramate dose. Results: We observed widespread edematous areas and necrotic cells in the islets of Langerhans in the MTX. We found that necrotic cells and edematous areas were decreased in the TPM compared to the MTX. We found fewer β cells showing insulin positivity in the MTX compared to the controls (p<0.05). We observed an increase in insulin-positive β-cells in the TPM compared to the MTX (p<0.05). We observed a significant decrease in the number of cells showing glucagon positivity in the MTX compared to the controls (p<0.05). We observed an increased number of α cells showing glucagon positivity in the TPM compared to the MTX (p<0.05). We found that the HDS increased from 0(0-1) in the control group to 6(6-8) in the MTX (p<0.05). It was found as 2(2-3) in the TPM (p<0.05). Conclusion: Our study shows that topiramate has a protective ef fect on pancreatic islet cells.  06 08 2023 94 91 10.30733/std.2023.01643 https://selcukmedj.org/en-us/the-effects-of-topiramate-on-methotrexate-induced-pancreatic-injury-en-7285/ https://app.selcukmedj.org/uploads/makale-pdf/2022-0096-943863543.pdf Metin Doğan SELİN UĞRAKLI Aim: Characterization of carbapenemase genes in K. pneumoniae isolates was aimed. Patients and Methods: In this study, 95 multi-drug resistant K. pneumoniae from samples between July 2016 and December 2017, were included. The MIC of meropenem, tigecycline and colistin were determined by Vitek 2, Etest and Broth Microdilution Methods (BMD). The Categorical agreement (CA), Very Major Error (VME) and Major Error (ME) rates were calculated and evaluated. The mcr-1, blaOXA-48, blaOXA-181, blaNDM-1, blaVIM, blaIMP genes were detected. Results: The meropenem resistance were determined in the isolates by BMD, Vitek 2 and Etest are respectively; as 70.5%, 87.4%, 81.1%. According to the BMD method, the rates of CA, VME and ME were determined by Vitek 2; Etest as 69.5%, 4.8% and 20 %; 70.5%, 1.5 % and 0 % respectively. It was determined that our isolates showed the highest sensitivity to tigecycline. The CA rates were determined by Vitek 2 and Etest for tigecycline as 70.5% and 95.8%. The ME rate determined by Vitek 2 was above the acceptable limit of 7.6%. The colistin resistance was 48.4% via BMD. The CA; VME; ME ratios determined by Vitek 2/Etest were 86.3%/72.6%; 17.4%/50%; 10.2%/6.1% respectively. In the isolates, predominantly blaOXA-48 (93.7%) was detected. blaOXA-48 was detected alone in 56. isolates and together with blaOXA-181 genes in 33 isolates. Lower colistin MIC levels were found in the only OXA-48 positive strains than in isolates together with OXA-181. The blaNDM-1 gene was investigated in 3.2% of the isolates. PCR and carbapenemase combined disc results were found to be compatible in 91.3% of the isolates. Conclusion: The blaOXA-48 gene region remains prevalent. In addition, comprehensive identification of the real prevalence and resistance dynamics of blaOXA-48-like gene regions will contribute to the development of national carbapenemase surveillance policies. 06 08 2023 75 83 10.30733/std.2023.01639 https://selcukmedj.org/en-us/phenotypic-and-genotypic-identification-of-carbapenem-resistant-klebsiella-pneumoniae-and-determination-of-antibiotic-susceptibili-en-7281/ https://app.selcukmedj.org/uploads/makale-pdf/2022-65-546466625.pdf ömer buhşem https://orcid.org/0000-0002-6438-6681 Abstract Aim: This study was designed to compare the effect of the classical bull horn excision pattern lip lift technique (group I) and the lip suspension technique (group II) which was performed using a dermal flap prepared on the upper lip with the same pattern incision, on patient satisfaction and scar appearance in lip lift surgeries. Patients and Methods: Twenty-eight female patients aged 48-66 years, operated by the author between 2016 and 2023, were analyzed in two groups. The first group included 14 patients who were operated on with the classical bull horn excision pattern lip lift technique, and the second group included 14 patients who were operated with the lip suspension technique using a dermal flap prepared on the upper lip with the same pattern incision under local anesthesia. At the end of the 6th month, the patients in both groups, who were followed up for 6 months, were asked about their satisfaction with the overall aesthetic result using the global aesthetic improvement scale. In addition, an evaluator surgeon scored the postoperative scar using the Vancouver scar scale. All results were evaluated statistically. Results: 9 of 14 patients (66%) stated very much improvement in the group 2 in which the dermal suspension flap was used, while 6 of 14 patients (%42.9) stated very much improvement with classical technique in Group 1, in the postoperative assessment at 6th month. Likewise, when the scar score performed by the evaluator surgeon was examined in 6th month. The scar scale was 14 in group 1 and 4 in group 2. In Group 1, the values of the patients ranged from 0 to 4, and the mean value was 1.0. In Group 2, the values ranged between 0-2 and the mean value was 0.3. Overall patient satisfaction was clinically significantly higher in group 2 when compared with group 1 and the scar was found to be clinically significantly less in group 2 when compared with group 1, even though there is no statistical significance (p>0.05) between 2 groups in overall patient satisfaction and scar score, probably due to limited number of patients in groups. Conclusion: It has been concluded that lip lift surgeries performed using the dermal suspension flap technique can contribute as a valuable alternative to the classical excision method, due to both higher overall aesthetic patient satisfaction and less scarring in our patients. 06 08 2023 69 74 10.30733/std.2023.01600 https://selcukmedj.org/en-us/lip-lift-wih-dermal-suspension-flap-technique-effect-on-patient-satisfaction-and-scar-appearance-en-7274/ https://app.selcukmedj.org/uploads/makale-pdf/1600-745938893.pdf Seda Ozcan Levent Elmas Uguray Hacısalihoglu Serdar Yanik sevinç şahin https://orcid.org/0000-0002-9409-8138 Aim: Glioblastoma is the most frequent, and fatal brain cancer in adults. Toll-like receptors are cell surface receptors comprised of 10 receptors (Toll-like receptor 1−10) related to triggering innate immunity by recognizing pathogens. However, some studies suggested that the expression of Toll-like receptors might be related to cancer cell proliferation and progression in some tumors including glioblastoma with some contradictory results. Thus, we aimed to investigate all ten members of the Toll-like receptor expression profile in glioblastoma for the first time in the literature to contribute additional data to the literature. Patients and Methods: Quantitative real-time polymerase chain reaction was applied to formalin-fixed paraffin-embedded tissues of 25 glioblastoma patients, diagnosed between January and December 2018, to evaluate the mRNA expression of Toll-like receptors. Also, the expression of each Toll-like receptor was investigated by cell culture analysis using five different cell lines of human glioblastoma (T98G, U87-MG, U373, LN18, and A172). The results were compared statistically. Results: Toll-like receptor 1, Toll-like receptor 6, and Toll-like receptor 7 mRNA levels were significantly increased in the formalin-fixed paraffin-embedded tissues of the glioblastoma group (p<0.001, each) whereas the expression of Toll-like receptor 4 and Toll-like receptor 10 was downregulated compared to the control group (p=0.023, p<0.001, respectively), by qPCR analysis.  Additionally, Toll-like receptor mRNA expression profiles differed among the cell lines. Conclusion: In our study, many Toll-like receptor members seemed to display different expression level in the glioblastoma microenvironment and affect it diversely.  Further comprehensive studies are required to confirm the endogenous protein level of each Toll-like receptor in glioblastoma, to clarify their precise role in the pathogenesis and prognosis of glioblastoma, and to shed light on new target therapies. 06 08 2023 62 68 10.30733/std.2023.01603 https://selcukmedj.org/en-us/investigation-of-toll-like-receptor-family-expression-in-glioblastoma-a-comparative-analysis-of-qpcr-and-cell-culture-en-7269/ https://app.selcukmedj.org/uploads/makale-pdf/1603-737096833.pdf neşe Arslan huri sabur alperen bahar https://orcid.org/0000-0001-5525-5551 06 08 2023 57 61 10.30733/std.2023.01580 https://selcukmedj.org/en-us/effects-of-contact-lens-wearing-habits-on-ocular-surface-and-microbial-flora-en-7264/ https://app.selcukmedj.org/uploads/makale-pdf/1580-633248290.pdf Yasemin Gönül MİTAT ARICIGİL Pembe OLTULU miyase orhan https://orcid.org/0000-0001-9449-3720 Purpose: Laryngeal squamous cell carcinoma poses a management challenge due to the lack of reliable prognostic markers. Although tumor budding (TB) has been shown to predict poor prognosis in some malignancies, its prognostic significance in laryngeal cancer remains uncertain in the literature. Therefore, the objective of this study is to evaluate the impact of TB on prognosis and its correlation with other established prognostic factors in laryngeal squamous cell carcinoma. Patients and Methods: In the department of otolaryngology the files of 60 patients with laryngeal squamous cell carcinoma who underwent surgery, postoperative chemoradiotherapy between 2008 and 2015 were analyzed retrospectively. The patient’s history, family history, age, TNM (tumor, node, metastases) classification, radiological imaging, type of surgery performed, and the results of the pathological specimen were evaluated. PanCK immunohistochemical staining was performed on old paraffin block sections containing tumoral tissue, previously stained with Hematoxylin & Eosin. The TB scores were evaluated by the pathology department, and the association between all obtained parameters and clinicopathological variables was analyzed. Results: Our findings showed a significant association between tumor budding and perineural infiltration, a known prognostic factor for laryngeal carcinoma (P=0.006). TB was found to be an independent risk factor for perineural infiltration and pathological lymph node involvement (p=0.003). Pathological lymph node involvement was also found to be an independent risk factor for lymphovascular invasion (p=0.028). Conclusions: Our study provides evidence for a significant association between tumor budding and perineural infiltration, which are established prognostic factors in laryngeal carcinoma. This suggests that tumor budding may be an important factor in determining tumor prognosis. 06 08 2023 51 56 10.30733/std.2023.01609 https://selcukmedj.org/en-us/prognostic-importance-of-tumor-buddings-in-larynx-squamous-cell-carcinomas-en-7257/ https://app.selcukmedj.org/uploads/makale-pdf/1609-411749158.pdf Sultan Kavuncuoğlu Mahmut Tarakçıoğlu Müge Payaslı Esin Aldemir Ozge Kucur https://orcid.org/0000-0002-6597-4549 Background: We aimed to investigate the neurodevelopmental outcomes and school success of 11- to 12-year-old children born as moderate/late preterm infants and identify risk factors affecting prognosis. Methods: Moderate/late preterm infants followed in the neonatal intensive care unit between January 2004 and December 2004 were included, and the children were examined again in our pediatrics outpatient clinic in 2016. Perinatal and neonatal histories were obtained from the hospital database. Physical growth characteristics were interpreted. Neurodevelopment was evaluated using the revised Wechsler Intelligence Scale for Children (WISC-R). The Pediatric Symptom Checklist (PSC) was also applied. The effect of socioeconomic level on neurodevelopmental outcome was examined. School performance was evaluated using report card grades. Results: Forty-one children with a mean age of 11.6 years were evaluated. Risk factors associated with physical growth outcomes were maternal age of >35 years, fetal distress, and patent ductus arteriosus. Sepsis was associated with a decrease in verbal intelligence while periventricular leukomalacia had negative effects on both verbal and performance intelligence. Socioeconomic level showed a medium correlation with performance and full-scale intelligence. The intelligence quotients of the children with positive PSC scores were significantly lower. Conclusions: Moderate/late preterm infants lag both neurologically and developmentally due to incomplete maturation of the brain and natal problems. Similarly, to early preterm infants, these children should be monitored for extended periods, and family support, rehabilitation, and special education needs should be met. 06 08 2023 44 50 10.30733/std.2023.01597 https://selcukmedj.org/en-us/neurodevelopmental-outcomes-of-moderatelate-preterm-infants-at-11-12-years-of-age-en-7245/ https://app.selcukmedj.org/uploads/makale-pdf/1597-622089833.pdf Salih Bölük SÜMEYRA BÖLÜK https://orcid.org/0000-0003-3906-6827 Intestinal intussusceptions are one of the causes of mechanical intestinal obstruction usually seen in childhood. Approximately 1-5% of invaginations seen in adults. While 90% of childhood cases are idiopathic, 90% of adult intussusceptions have an underlying organic lesion. We aimed to give information about intussusception cases in adults with 2 cases we present . 06 08 2023 92 96 10.30733/std.2021.01637 https://selcukmedj.org/en-us/intestinal-intussusception-seen-in-adult-patients-case-report-en-7277/ https://app.selcukmedj.org/uploads/makale-pdf/2022-113-713582578.pdf